23 February 2013 Effects of nano-second electrical pulses (nsPEFs) on cell cycle progression and susceptibility at various phases
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Abstract
Exposure to nano-second pulsed electrical fields (nsPEFs) has been shown to cause poration of external and internal cell membranes, DNA damage, and blebbing of the plasma membrane. Recovery from nsPEF exposure is likely dependent on multiple factors, including exposure parameters, length of time between pulses, and extent of cellular damage. As cells progress through the cell cycle, variations in DNA and nucleus structure, cytoskeletal arrangement, and elasticity of cell membrane could cause nsPEFs to affect cells differently during different cell cycle phases. To better understand the impact of nsPEF on cell cycle, we investigated CHO cell cycle progression following varying intensities of nsPEFexposures. Cell populations were examined post exposure (10 ns pulse trains at 100, 150, or 200kV/cm) by analysis of DNA content via propidium iodide staining and flow cytometric analysis to determine cell cycle phase. Populations exhibited arrest in G2/M phase, but not in G1 phase at 1h post-exposure that increased in severity and duration with increasing exposure dose. Recovery from arrest was complete after 12h, and populations did not exhibit an increase in apoptosis as a result of exposure. Post exposure arrest in G2/M phase may indicate that nsPEF-induced damage is not significant to cause G1 arrest or that mitotic checkpoints are more important regulators of cell cycle progression after nsPEF exposure.
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Megan A. Mahlke, Megan A. Mahlke, Gary Thompson, Gary Thompson, Larry Estlack, Larry Estlack, Christopher Navara, Christopher Navara, Bennett L. Ibey, Bennett L. Ibey, } "Effects of nano-second electrical pulses (nsPEFs) on cell cycle progression and susceptibility at various phases", Proc. SPIE 8585, Terahertz and Ultrashort Electromagnetic Pulses for Biomedical Applications, 85850O (23 February 2013); doi: 10.1117/12.2020679; https://doi.org/10.1117/12.2020679
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