Development of optics with micro-LED arrays for improved opto-electronic neural stimulation

Lionel Chaudet; Mark Neil; Patrick Degenaar; Kamyar Mehran; Rolando Berlinguer-Palmini; Brian Corbet; Pleun Maaskant; David Rogerson; Peter Lanigan; Ernst Bamberg; Botond Roska

doi:10.1117/12.2002070

8 March 2013 Development of optics with micro-LED arrays for improved opto-electronic neural stimulation

Lionel Chaudet, Mark Neil, Patrick Degenaar, Kamyar Mehran, Rolando Berlinguer-Palmini , Brian Corbet, Pleun Maaskant, David Rogerson, Peter Lanigan, Ernst Bamberg, Botond Roska

Author Affiliations +

Proceedings Volume 8586, Optogenetics: Optical Methods for Cellular Control; 85860R (2013) https://doi.org/10.1117/12.2002070
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The breakthrough discovery of a nanoscale optically gated ion channel protein, Channelrhodopsin 2 (ChR2), and its combination with a genetically expressed ion pump, Halorhodopsin, allowed the direct stimulation and inhibition of individual action potentials with light alone. This work reports developments of ultra-bright elec tronically controlled optical array sources with enhanced light gated ion channels and pumps for use in systems to further our understanding of both brain and visual function. This work is undertaken as part of the European project, OptoNeuro. Micro-LED arrays permit spatio-temporal control of neuron stimulation on sub-millisecond timescales. However they are disadvantaged by their broad spatial light emission distribution and low fill factor. We present the design and implementation of a projection and micro-optics system for use with a micro-LED array consisting of a 16x16 matrix of 25 μm diameter micro-LEDs with 150 μm centre-to-centre spacing and an emission spectrum centred at 470 nm overlapping the peak sensitivity of ChR2 and its testing on biological samples. The projection system images the micro-LED array onto micro-optics to improve the fill-factor from ~2% to more than 78% by capturing a larger fraction of the LED emission and directing it correctly to the sample plane. This approach allows low fill factor arrays to be used effectively, which in turn has benefits in terms of thermal management and electrical drive from CMOS backplane electronics. The entire projection system is integrated into a microscope prototype to provide stimulation spots at the same size as the neuron cell body (μ10 pm).

Citation Download Citation

Lionel Chaudet, Mark Neil, Patrick Degenaar, Kamyar Mehran, Rolando Berlinguer-Palmini , Brian Corbet, Pleun Maaskant, David Rogerson, Peter Lanigan, Ernst Bamberg, and Botond Roska "Development of optics with micro-LED arrays for improved opto-electronic neural stimulation ", Proc. SPIE 8586, Optogenetics: Optical Methods for Cellular Control, 85860R (8 March 2013); https://doi.org/10.1117/12.2002070

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available