Label-free imaging of fatty acid content within yeast samples

N. Garrett; J. Moger

doi:10.1117/12.2002970

22 February 2013 Label-free imaging of fatty acid content within yeast samples

N. Garrett, J. Moger

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85870O (2013) https://doi.org/10.1117/12.2002970
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Fungi have been found to be an underlying cause of 70% of all plant and animal extinctions caused by infectious diseases. Fungal infections are a growing problem affecting global health, food production and ecosystems. Lipid metabolism is a promising target for antifungal drugs and since effective treatment of fungal infections requires a better understanding of the effects of antifungal agents at the cellular level, new techniques are needed to investigate this problem. Recent advances in nonlinear microscopy allow chemically-specific contrast to be obtained non-invasively from intrinsic chemical bonds within live samples using advanced spectroscopy techniques probing Raman-active resonances. We present preliminary data using Stimulated Raman Scattering (SRS) microscopy as a means to visualise lipid droplets within individual living fungi by probing Raman resonances of the CH stretching region between 2825cm^-1 and 3030cm^-1.

Citation Download Citation

N. Garrett and J. Moger "Label-free imaging of fatty acid content within yeast samples", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85870O (22 February 2013); https://doi.org/10.1117/12.2002970

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