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22 February 2013 In vivo skin chromophore mapping using a multimode imaging dermoscope (SkinSpec™)
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We introduce a multimode dermoscope (SkinSpectTM) we developed for early detection of melanoma by combining fluorescence, polarization and hyperspectral imaging. Acquired reflection image datacubes were input to a wavelength-dependent linear model to extract the relative contributions of skin chromophores at every pixel. The oxy-hemoglobin, deoxy hemoglobin, melanin concentrations, and hemoglobin oxygen saturation by the single step linear least square fitting and Kubelka-Munk tissue model using cross polarization data cubes were presented. The comprehensive data obtained by SkinSpect can be utilized to improve the accuracy of skin chromophore decomposition algorithm with less computation cost. As an example in this work, the deoxy-hemoglobin over-estimation error in highly pigmented lesion due to melanin and deoxy hemoglobin spectral cross talk were analyzed and corrected using two-step linear least square fitting procedure at different wavelength ranges. The proposed method also tested in skin with underlying vein area for validating the proof of concept.
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Nicholas MacKinnon, Fartash Vasefi, Eugene Gussakovsky, Gregory Bearman, Robert Chave, and Daniel L. Farkas "In vivo skin chromophore mapping using a multimode imaging dermoscope (SkinSpec™)", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85870U (22 February 2013);

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