Multi-spectral analysis of animal tissues in the second NIR window based on endogenous chromophores

Qian Cao; Steven T. Wang; Natalia G. Zhegalova; Walter J. Akers; Mikhail Y. Berezin

doi:10.1117/12.2008786

22 February 2013 Multi-spectral analysis of animal tissues in the second NIR window based on endogenous chromophores

Qian Cao, Steven T. Wang, Natalia G. Zhegalova, Walter J. Akers, Mikhail Y. Berezin

Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85870V (2013) https://doi.org/10.1117/12.2008786
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

To minimize the problem with scattering in deep tissues while increasing the penetration depth, we explored the feasibility of imaging in the previously unexplored extended NIR (exNIR) spectral region at 900 - 1400 nm with endogenous chromophores. This region, also known as second NIR window, is weakly dominated by absorption of water and lipids and free from other endogenous chromophores, with virtually no autofluorescence. To demonstrate the applicability of the exNIR in bioimaging, we analyzed optical properties of individual components and animal organs using InGaAs spectrophotometer and a multispectral InGaAs scanning imager featuring in transmission geometry.

Citation Download Citation

Qian Cao, Steven T. Wang, Natalia G. Zhegalova, Walter J. Akers, and Mikhail Y. Berezin "Multi-spectral analysis of animal tissues in the second NIR window based on endogenous chromophores", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85870V (22 February 2013); https://doi.org/10.1117/12.2008786

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available