Quantifying the optical properties and chromophore concentrations of
turbid media using polarization sensitive hyperspectral imaging:
optical phantom studies

Fartash Vasefi; Rolf Saager; Anthony J. Durkin; Nicholas MacKinnon; Eugene Gussakovsky; Robert Chave; Daniel L. Farkas

doi:10.1117/12.2005371

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26 February 2013 Quantifying the optical properties and chromophore concentrations of turbid media using polarization sensitive hyperspectral imaging: optical phantom studies

Fartash Vasefi; Rolf Saager; Anthony J. Durkin; Nicholas MacKinnon; Eugene Gussakovsky; Robert Chave; Daniel L. Farkas

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Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85870Z (2013) https://doi.org/10.1117/12.2005371
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We present a polarization-sensitive hyperspectral imaging system (SkinSpect) that employs a spectrally-programmable light source in the visible and NIR domains. Multiple tissue-mimicking phantoms were fabricated to mimic the optical properties of normal skin as well as pigmented light and dark moles. The phantoms consist of titanium dioxide and a mixture of coffee, red food dye, and naphthol green as the scattering and the three absorptive agents in a polydimethylsiloxane (PDMS) base. Phantoms were produced with both smooth and rough textured surfaces and tested using Spatial Frequency Domain Imaging (SFDI) and Spatially Modulated Quantitative Spectroscopy (SMoQS) for homogeneity as well as determining absorption and scattering variance, respectively. The reflectance spectral images were also recorded using the SkinSpect research prototype; the spectral signatures of the phantoms were calculated using a two-flux single-layer Kubelka-Munk model and non-negative least square fitting routine was applied to extract the relative concentrations of the individual phantom components.

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Fartash Vasefi, Rolf Saager, Anthony J. Durkin, Nicholas MacKinnon, Eugene Gussakovsky, Robert Chave, and Daniel L. Farkas "Quantifying the optical properties and chromophore concentrations of turbid media using polarization sensitive hyperspectral imaging: optical phantom studies", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85870Z (26 February 2013); https://doi.org/10.1117/12.2005371

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