26 February 2013 Disassembly of myofibrils in adult cardiomyocytes during dedifferentiation
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Abstract
Using hybrid TPEF-SHG imaging and immunocytological techniques, we studied dedifferentiation of adult cardiomyocytes. First, the myofibrils shrank to shorten the sarcomere length. At the cell ends, the striated pattern of myosin filaments began to dissociate; at the center of the cell, the striated pattern of alpha-actinin first faded away and reappeared near the cell membrane during dedifferentiation. The results suggest that when freshly isolated adult cardiomyocytes are used to model cardiac muscle, the end-to-end connection may be important to maintain their striated myofibrillar structure and rod-shape morphology.
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Honghai Liu, Honghai Liu, Wan Qin, Wan Qin, Yonghong Shao, Yonghong Shao, Zhonghai Wang, Zhonghai Wang, Huaxiao Yang, Huaxiao Yang, Raymond B. Runyan, Raymond B. Runyan, Thomas K. Borg, Thomas K. Borg, Bruce Gao, Bruce Gao, } "Disassembly of myofibrils in adult cardiomyocytes during dedifferentiation", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871W (26 February 2013); doi: 10.1117/12.2005156; https://doi.org/10.1117/12.2005156
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