22 February 2013 Study of virus by Raman spectroscopy
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Abstract
Problem of viruses is very actual for nowadays. Some viruses, which are responsible for human of all tumors, are about 15 %. Main purposes this study, early detection virus in live cell without labeling and in the real time by Raman spectroscopy. Micro Raman spectroscopy (mRs) is a technique that uses a Raman spectrometer to measure the spectra of microscopic samples. According to the Raman spectroscopy, it becomes possible to study the metabolites of a live cultured cell without labeling. We used mRs to detect the virus via HEK 293 cell line-infected adenovirus. We obtained raman specters of lives cells with viruses in 24 hours and 7 days after the infection. As the result, there is some biochemical changing after the treatment of cell with virus. One of biochemical alteration is at 1081 cm-1. For the clarification result, we use confocal fluorescent microscopy and transmission electron microscopy (TEM).
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K. Moor, K. Moor, H. Kitamura, H. Kitamura, K. Hashimoto, K. Hashimoto, M. Sawa, M. Sawa, B. B. Andriana, B. B. Andriana, K. Ohtani , K. Ohtani , T. Yagura, T. Yagura, H. Sato, H. Sato, } "Study of virus by Raman spectroscopy", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871X (22 February 2013); doi: 10.1117/12.2004476; https://doi.org/10.1117/12.2004476
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