Synthesize dye-bioconjugates to visualize cancer cells using fluorescence microscopy

Yang Pu; Rui Tang; Jianpeng Xue; W. B. Wang; Baogang Xu; Duanwen Shen; Sharon Bloch; Mingzhou Zhou; S. Achilefu; R. R. Alfano

doi:10.1117/12.2000080

22 February 2013 Synthesize dye-bioconjugates to visualize cancer cells using fluorescence microscopy

Yang Pu, Rui Tang, Jianpeng Xue, W. B. Wang, Baogang Xu, Duanwen Shen, Sharon Bloch, Mingzhou Zhou, S. Achilefu, R. R. Alfano

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Proceedings Volume 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI; 85871Z (2013) https://doi.org/10.1117/12.2000080
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The clinical diagnosis of most cancers is based on evaluation of histology microscopic slide to view the size and shape of cellular nuclei, and morphological structure of tissue. To achieve this goal in vivo and in deep tissue, near infrared (NIR) dyes-bovine serum albumin (BSA) and immunoglobulin G (IgG) conjugates were synthesized. The spectral study show that the absorption and fluorescence of the dye-conjugates are in the “tissue optical window” between 650 nm and 1100 nm. The internalization and pinocytosis of the synthesized compound were investigated in cell level using fluorescence microscopy to obtain the optimal concentration and staining time scale.

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Yang Pu, Rui Tang, Jianpeng Xue, W. B. Wang, Baogang Xu, Duanwen Shen, Sharon Bloch, Mingzhou Zhou, S. Achilefu, and R. R. Alfano "Synthesize dye-bioconjugates to visualize cancer cells using fluorescence microscopy", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871Z (22 February 2013); https://doi.org/10.1117/12.2000080

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KEYWORDS

Luminescence

Near infrared

Absorption

Microscopy

Cancer

Tissue optics

Tissues

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