22 February 2013 Synthesize dye-bioconjugates to visualize cancer cells using fluorescence microscopy
Author Affiliations +
Abstract
The clinical diagnosis of most cancers is based on evaluation of histology microscopic slide to view the size and shape of cellular nuclei, and morphological structure of tissue. To achieve this goal in vivo and in deep tissue, near infrared (NIR) dyes-bovine serum albumin (BSA) and immunoglobulin G (IgG) conjugates were synthesized. The spectral study show that the absorption and fluorescence of the dye-conjugates are in the “tissue optical window” between 650 nm and 1100 nm. The internalization and pinocytosis of the synthesized compound were investigated in cell level using fluorescence microscopy to obtain the optimal concentration and staining time scale.
© (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yang Pu, Yang Pu, Rui Tang, Rui Tang, Jianpeng Xue, Jianpeng Xue, W. B. Wang, W. B. Wang, Baogang Xu, Baogang Xu, Duanwen Shen, Duanwen Shen, Sharon Bloch, Sharon Bloch, Mingzhou Zhou, Mingzhou Zhou, S. Achilefu, S. Achilefu, R. R. Alfano, R. R. Alfano, } "Synthesize dye-bioconjugates to visualize cancer cells using fluorescence microscopy", Proc. SPIE 8587, Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues XI, 85871Z (22 February 2013); doi: 10.1117/12.2000080; https://doi.org/10.1117/12.2000080
PROCEEDINGS
8 PAGES


SHARE
Back to Top