22 February 2013 Spectroscopic SRS imaging with a time-lens source synchronized to a femtosecond pulse shaper
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Though single-color coherent Raman microscopy has been widely used for vibrational imaging of isolated Raman bands, it is still challenging to visualize molecules having overlapping Raman bands. We address this issue by developing a spectroscopic SRS microscope with a time-lens laser source synchronized to a femtosecond laser. The time-lens source provides 2-ps pulse at the wavelength of 1064 nm. A pulse shaper is installed for intra-pulse spectral scanning of the femtosecond laser output. By electronically modulating the time-lens source at MHz frequency, spectroscopic stimulated Raman loss (SRL) images were obtained on a laser-scanning microscope. Using this microscope, we have been able to detect 0.2% DMSO in aqueous solution. Spectroscopic SRL images of prostate cancer cells were obtained. Multivariate curve resolution analysis was further applied to decompose the SRL images into concentration maps of proteins and lipids. With high sensitivity and high spectral resolution, this method offers exciting potential in label-free imaging of live cells using fingerprint Raman bands.
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Ke Wang, Ke Wang, Delong Zhang, Delong Zhang, Kriti Charan, Kriti Charan, Mikhail N. Slipchenko, Mikhail N. Slipchenko, Ping Wang, Ping Wang, Ji-Xin Cheng, Ji-Xin Cheng, Chris Xu, Chris Xu, } "Spectroscopic SRS imaging with a time-lens source synchronized to a femtosecond pulse shaper", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85880B (22 February 2013); doi: 10.1117/12.2004905; https://doi.org/10.1117/12.2004905

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