22 February 2013 Combining multiphoton and CARS microscopy for skin imaging
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Microscopic imaging based on multiphoton fluorescence, second harmonic generation (SHG) and coherent anti-Stokes Raman scattering (CARS) imaging has been realized in one common platform which is appropriate for use in hospitals. The different optical modalities non-invasively provide in vivo images from human skin with subcellular resolution, at different depths based on endogenous fluorescent, SHG-active molecules as well as non-fluorescent molecules with vibrational resonances at 2845 cm-1, in particular lipids. An overview of the system employing a Ti:sapphire laser and photonic crystal fiber to generate the excitation light as well as several imaging examples are presented.
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H. G. Breunig, M. Weinigel, M. Kellner-Höfer, R. Bückle, M. E. Darvin, J. Lademann, K. König, "Combining multiphoton and CARS microscopy for skin imaging", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85880N (22 February 2013); doi: 10.1117/12.2001985; https://doi.org/10.1117/12.2001985

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