Optical clearing and multiphoton imaging of paraffin-embedded specimens

Jesse W. Wilson; Simone Degan; Martin C. Fischer; Warren S. Warren

doi:10.1117/12.2003155

22 February 2013 Optical clearing and multiphoton imaging of paraffin-embedded specimens

Jesse W. Wilson, Simone Degan, Martin C. Fischer, Warren S. Warren

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 858813 (2013) https://doi.org/10.1117/12.2003155
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

New labeling, imaging, or analysis tools could provide new retrospective insights when applied to archived, paraffin-embedded samples. Deep-tissue multiphoton microscopy of paraffin-embedded specimens is achieved using optical clearing with mineral oil. We tested a variety of murine tissue specimens including skin, lung, spleen, kidney, and heart, acquiring multiphoton autofluorescence and second-harmonic generation, and pump-probe images This technique introduces the capability for non-destructive 3-dimensional microscopic imaging of existing archived pathology specimens, enabling retrospective studies.

Citation Download Citation

Jesse W. Wilson, Simone Degan, Martin C. Fischer, and Warren S. Warren "Optical clearing and multiphoton imaging of paraffin-embedded specimens", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858813 (22 February 2013); https://doi.org/10.1117/12.2003155

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