22 February 2013 Optical clearing and multiphoton imaging of paraffin-embedded specimens
Author Affiliations +
Abstract
New labeling, imaging, or analysis tools could provide new retrospective insights when applied to archived, paraffin-embedded samples. Deep-tissue multiphoton microscopy of paraffin-embedded specimens is achieved using optical clearing with mineral oil. We tested a variety of murine tissue specimens including skin, lung, spleen, kidney, and heart, acquiring multiphoton autofluorescence and second-harmonic generation, and pump-probe images This technique introduces the capability for non-destructive 3-dimensional microscopic imaging of existing archived pathology specimens, enabling retrospective studies.
© (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jesse W. Wilson, Jesse W. Wilson, Simone Degan, Simone Degan, Martin C. Fischer, Martin C. Fischer, Warren S. Warren, Warren S. Warren, } "Optical clearing and multiphoton imaging of paraffin-embedded specimens", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858813 (22 February 2013); doi: 10.1117/12.2003155; https://doi.org/10.1117/12.2003155
PROCEEDINGS
6 PAGES


SHARE
Back to Top