22 February 2013 Optical clearing and multiphoton imaging of paraffin-embedded specimens
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New labeling, imaging, or analysis tools could provide new retrospective insights when applied to archived, paraffin-embedded samples. Deep-tissue multiphoton microscopy of paraffin-embedded specimens is achieved using optical clearing with mineral oil. We tested a variety of murine tissue specimens including skin, lung, spleen, kidney, and heart, acquiring multiphoton autofluorescence and second-harmonic generation, and pump-probe images This technique introduces the capability for non-destructive 3-dimensional microscopic imaging of existing archived pathology specimens, enabling retrospective studies.
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Jesse W. Wilson, Jesse W. Wilson, Simone Degan, Simone Degan, Martin C. Fischer, Martin C. Fischer, Warren S. Warren, Warren S. Warren, "Optical clearing and multiphoton imaging of paraffin-embedded specimens", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858813 (22 February 2013); doi: 10.1117/12.2003155; https://doi.org/10.1117/12.2003155

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