High contrast in vivo bioimaging using multiphoton upconversion in novel rare-earth-doped fluoride upconversion nanoparticles

Guanying Chen; Chunhui Yang; Paras N. Prasad

doi:10.1117/12.2000112

22 February 2013 High contrast in vivo bioimaging using multiphoton upconversion in novel rare-earth-doped fluoride upconversion nanoparticles

Guanying Chen, Chunhui Yang, Paras N. Prasad

Author Affiliations +

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 858814 (2013) https://doi.org/10.1117/12.2000112
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Upconversion in rare-earth ions is a sequential multiphoton process that efficiently converts two or more low-energy photons, which are generally near infrared (NIR) light, to produce anti-Stokes emission of a higher energy photon (e.g., NIR, visible, ultraviolet) using continuous-wave (cw) diode laser excitation. Here, we show the engineering of novel, efficient, and biocompatible NIR_in-to-NIR_out upconversion nanoparticles for biomedical imaging with both excitation and emission being within the “optical transparency window” of tissues. The small animal whole-body imaging with exceptional contrast (signal-to-noise ratio of 310) was shown using BALB/c mice intravenously injected with aqueously dispersed nanoparticles. An imaging depth as deep as 3.2-cm was successfully demonstrated using thick animal tissue (pork) under cw laser excitation at 980 nm.

Citation Download Citation

Guanying Chen, Chunhui Yang, and Paras N. Prasad "High contrast in vivo bioimaging using multiphoton upconversion in novel rare-earth-doped fluoride upconversion nanoparticles", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858814 (22 February 2013); https://doi.org/10.1117/12.2000112

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