22 February 2013 Multiphoton cryo microscope with sample temperature control
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We present a multiphoton microscope system which combines the advantages of multiphoton imaging with precise control of the sample temperature. The microscope provides online insight in temperature-induced changes and effects in plant tissue and animal cells with subcellular resolution during cooling and thawing processes. Image contrast is based on multiphoton fluorescence intensity or fluorescence lifetime in the range from liquid nitrogen temperature up to +600°C. In addition, micro spectra from the imaged regions can be recorded. We present measurement results from plant leaf samples as well as Chinese hamster ovary cells.
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H. G. Breunig, H. G. Breunig, A. Uchugonova, A. Uchugonova, K. König, K. König, "Multiphoton cryo microscope with sample temperature control", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858819 (22 February 2013); doi: 10.1117/12.2001994; https://doi.org/10.1117/12.2001994

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