Hierarchical model of fibrillar collagen distribution for polarization-resolved SHG microscopy

Adam E. Tuer; Margarete K. Akens; Serguei Krouglov; Daaf Sandkuijl; Brian C. Wilson; Cari M. Whyne; Virginijus Barzda

doi:10.1117/12.2000724

22 February 2013 Hierarchical model of fibrillar collagen distribution for polarization-resolved SHG microscopy

Adam E. Tuer, Margarete K. Akens, Serguei Krouglov, Daaf Sandkuijl, Brian C. Wilson, Cari M. Whyne, Virginijus Barzda

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85881O (2013) https://doi.org/10.1117/12.2000724
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

A hierarchical model of the organization of fibrillar collagen is developed and its implications on polarization-resolved second harmonic generation (SHG) microscopy are investigated. A “ground-up” approach is employed to develop the theory for understanding of the origin of SHG from fibrillar collagen. The effects of fibril ultrastructure and fibril macroscopic organization on the second-order polarization properties of fibrillar collagen are presented in conjunction with recent ab initio results performed on a collagen triple-helix model (-GLY-PRO-HYP-)_n. Various tissues containing fibrillar collagen are quantified using a polarization-resolved SHG technique, termed polarization-in, polarization-out (PIPO) and interpreted in light of the aforementioned theory. The method involves varying the incident laser polarization, while monitoring the SHG intensity through an analyzer. From the SHG polarization data the orientation of the fibers, in biological tissue, can be deduced. Unique PIPO signatures are observed for different rat tissues and interpreted in terms of the collagen composition, fibril ultrastructure, and macroscopic organization. Similarities and discrepancies in the second-order polarization properties of different collagen types and ultrastructures will be presented. PIPO SHG microscopy shows promise in its ability to quantify the organization of collagen in various tissues. The ability to characterize the structure of collagen in various tissue microenvironments will aid in the study of numerous collagen related biological process, including tissue diseases, wound repair, and tumor development and progression.

Citation Download Citation

Adam E. Tuer, Margarete K. Akens, Serguei Krouglov, Daaf Sandkuijl, Brian C. Wilson, Cari M. Whyne, and Virginijus Barzda "Hierarchical model of fibrillar collagen distribution for polarization-resolved SHG microscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85881O (22 February 2013); https://doi.org/10.1117/12.2000724

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available