Multiphoton imaging to distinguish grana and starch  inside an intact leaf

Mei-Yu Chen; Guan-Yu Zhuo; Po-Fu Chen; Pei-Chun Wu; Tzu-Ming Liu; Shi-Wei Chu

doi:10.1117/12.2003075

22 February 2013 Multiphoton imaging to distinguish grana and starch inside an intact leaf

Mei-Yu Chen, Guan-Yu Zhuo, Po-Fu Chen, Pei-Chun Wu, Tzu-Ming Liu, Shi-Wei Chu

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 858822 (2013) https://doi.org/10.1117/12.2003075
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We have demonstrated a straightforward and noninvasive method to identify the distribution of grana and starch within an intact leaf. Grana and starch are the major functional structures for photosynthesis and energy storage of plant, respectively. Both exhibit highly ordered molecular structures and appear as micrometer-sized granules inside chloroplasts. In order to distinguish grana and starch, we used multiphoton microscopy, with simultaneous acquisition of two photon fluorescence (2PF) and second harmonic generation (SHG) signals. Consequently, SHG is found on both grana and starch while 2PF from chlorophyll indicates the identity of grana.

Citation Download Citation

Mei-Yu Chen, Guan-Yu Zhuo, Po-Fu Chen, Pei-Chun Wu, Tzu-Ming Liu, and Shi-Wei Chu "Multiphoton imaging to distinguish grana and starch inside an intact leaf", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858822 (22 February 2013); https://doi.org/10.1117/12.2003075

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KEYWORDS

Second-harmonic generation

Luminescence

Multiphoton microscopy

Harmonic generation

Microscopy

Photosynthesis

Signal generators

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