Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy

Nirmal Mazumder; Jianjun Qiu; Chih-Wei Hu; Fu-Jen Kao

doi:10.1117/12.2003894

22 February 2013 Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy

Nirmal Mazumder, Jianjun Qiu, Chih-Wei Hu, Fu-Jen Kao

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 858823 (2013) https://doi.org/10.1117/12.2003894
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We analyzed the polarization states of second harmonic generation (SHG) signals from starch granules and type I collagen through a four-channel photon counting based Stokes-polarimeter. The 2D SHG images of samples are reconstructed using various polarization parameters, such as the degree of polarization (DOP), the degree of linear polarization (DOLP), the degree of circular polarization (DOCP), as well as the anisotropy from the acquired Stokes parameters. Furthermore, we have demonstrated that the polarization parameters are changes at different input polarizations and focusing depths.

Citation Download Citation

Nirmal Mazumder, Jianjun Qiu, Chih-Wei Hu, and Fu-Jen Kao "Imaging molecular structure with Stokes-polarimeter based second harmonic generation microscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858823 (22 February 2013); https://doi.org/10.1117/12.2003894

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