Two-Photon Fluorescence Stereomicroscopy with Bessel Beams

Yanlong Yang; Ming Lei; Juanjuan Zheng; Runze Li; Shaohui Yan; Baoli Yao; Tong Ye

doi:10.1117/12.2002850

22 February 2013 Two-photon fluorescence stereomicroscopy with Bessel beams

Yanlong Yang, Ming Lei, Juanjuan Zheng, Runze Li, Shaohui Yan, Baoli Yao, Tong Ye

Proceedings Volume 8588, Multiphoton Microscopy in the Biomedical Sciences XIII; 85882K (2013) https://doi.org/10.1117/12.2002850
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Three dimensional distributions of cells can be usually acquired by optical sectioning methods, such as multiphoton excitation and confocal fluorescence laser scanning microscopy. Though the lateral scan rates can reach up to several kHz, the relatively slow axial scan comprises the speed of real-time imaging of a volume. Here we propose a three dimensional imaging method that uses Bessel beams as excitation in multiphoton fluorescence microscopy. The extended focus of the Bessel beam allows recording a volume of cells without scanning the depth. The depth information can be retrieved by recording a pair of parallax views of the same volume. We have demonstrated the stereoscope capability on a homebuilt two-photon fluorescence microscope.

Citation Download Citation

Yanlong Yang, Ming Lei, Juanjuan Zheng, Runze Li, Shaohui Yan, Baoli Yao, and Tong Ye "Two-photon fluorescence stereomicroscopy with Bessel beams", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882K (22 February 2013); https://doi.org/10.1117/12.2002850

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