22 February 2013 In vivo imaging of dermal collagen in skin burn by collagen-sensitive second-harmonic-generation microscopy
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Abstract
Optical assessment of skin burns is possible with second-harmonic-generation (SHG) microscopy due to its high sensitivity to thermal denaturation of collagen molecules. In contrast to previous studies that were performed using excised tissue specimens ex vivo, in this study, we demonstrated in vivo observation of dermal collagen fibers in living rat burn models with SHG microscopy. We confirmed that changes in SHG vanishing patterns in the SHG images depended on the burn degree. The results imply that SHG microscopy can be used as a low-invasiveness, highly quantitative tool for skin burn assessment.
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Takeshi Yasui, Ryosuke Tanaka, Eiji Hase, Shu-ichiro Fukushima, Tsutomu Araki, "In vivo imaging of dermal collagen in skin burn by collagen-sensitive second-harmonic-generation microscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882W (22 February 2013); doi: 10.1117/12.2002927; https://doi.org/10.1117/12.2002927
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