22 February 2013 In vivo imaging of collagen fiber orientation with rapid polarization-resolved SHG microscopy
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Abstract
Polarization-resolved second-harmonic-generation (SHG) microscopy is a powerful tool to visualize distribution of collagen fiber orientation in tissue with little invasion. However, long image acquisition time, resulting from mechanical rotation of a half-wave plate, makes this microscopy easy to suffer from motion artifact of a sample and hence has limited its use to in vivo application. In this paper, we constructed rapid, polarization-resolved SHG microscopy by combination of an electro-optics-modulator-based polarization modulation with improved data acquisition method. The constructed polarization-resolved SHG microscopy enables us to visualize orientation mapping of dermal collagen fiber in rat skin and human one in vivo without influence of motion artifact. This microscope will open the door for in vivo measurement of collagen fiber orientation in human skin.
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Yuji Tanaka, Yuji Tanaka, Eiji Hase, Eiji Hase, Shuichiro Fukushima, Shuichiro Fukushima, Takeshi Yasui, Takeshi Yasui, Tsutomu Araki, Tsutomu Araki, } "In vivo imaging of collagen fiber orientation with rapid polarization-resolved SHG microscopy", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 85882X (22 February 2013); doi: 10.1117/12.2003969; https://doi.org/10.1117/12.2003969
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