22 February 2013 Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission
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Abstract
We present here a stimulated emission based fluorescence lifetime imaging (FLIM) scheme using a pair of synchronized diode lasers operating at gain switched pulse mode. The two semiconductor lasers, with wavelengths at 635 nm and 700 nm, serve as the excitation and the stimulation light sources for the ATTO647N labeled sample, respectively. FLIM is readily achieved with their relative time delay controlled electronically. The coherent nature of the stimulated emission signal also allows FLIM at long working distance. In this way, a high performance all-semiconductor FLIM module is realized in a flexible, compact, and cost effective configuration.
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Jianhong Ge, Jianhong Ge, Yixin Wang, Yixin Wang, Cuifang Kuang, Cuifang Kuang, Shin-Shian Lee, Shin-Shian Lee, Nirmal Mazumder, Nirmal Mazumder, Fu-Jen Kao, Fu-Jen Kao, } "Fluorescence lifetime imaging with pulsed diode laser enabled stimulated emission", Proc. SPIE 8588, Multiphoton Microscopy in the Biomedical Sciences XIII, 858832 (22 February 2013); doi: 10.1117/12.2009387; https://doi.org/10.1117/12.2009387
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