GPU-based image registration in aperture correlation microscopy, and reflection mode correlation microscopy

Lionel J. Fafchamps; Mark A. A. Neil; Rimas Juskaitis

doi:10.1117/12.2004968

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22 February 2013 GPU-based image registration in aperture correlation microscopy, and reflection mode correlation microscopy

Lionel J. Fafchamps, Mark A. A. Neil, Rimas Juskaitis

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Proceedings Volume 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX; 858907 (2013) https://doi.org/10.1117/12.2004968
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Aperture Correlation Microscopy (ACM) is a fluorescence microscopy technique capable of depth resolved imaging and enhanced lateral resolution at real-time acquisition rates. It relies on the subtraction of 2 separate images from different cameras which must be registered to the sub-pixel level. In order to achieve real-time registration and subtraction, the graphics processing unit (GPU) is used to apply a transformation from one frame to the other, resulting in a system capable of processing over 200 frames per second on modest hardware (GeForce 330M). Currently, this rate is limited by camera acquision to 16fps. Additionally, a novel reflection mode correlation microscope is introduced which functions on similar principles as the fluorescent system but can be used to examine reflective samples. Images and z-stacks taken with this system are presented here.

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Lionel J. Fafchamps, Mark A. A. Neil, and Rimas Juskaitis "GPU-based image registration in aperture correlation microscopy, and reflection mode correlation microscopy", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858907 (22 February 2013); https://doi.org/10.1117/12.2004968

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