22 February 2013 Supercritical self-interference fluorescence microscopy for full-field membrane imaging
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Abstract
We present a new technique based on the self-interference of Supercritical Angle Fluorescence (SAF) emission in order to perform full-field cell membrane imaging. We show that our Point Spread Function (PSF) engineering technique allows us to obtain a 100 nm axial sectioning while conserving the original lateral resolution of the microscope. The images are acquired using an optical module that can be connected to any fluorescent microscope to simultaneously monitor in real time both the cell membrane and in-depth phenomena.
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Thomas Barroca, Pierre Bon, Sandrine Lévêque-Fort, Emmanuel Fort, "Supercritical self-interference fluorescence microscopy for full-field membrane imaging", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858911 (22 February 2013); doi: 10.1117/12.2003736; https://doi.org/10.1117/12.2003736
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