Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope

Pierre Bon; Shérazade Aknoun; Julien Savatier; Benoit Wattellier; Serge Monneret

doi:10.1117/12.2003426

22 February 2013 Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope

Pierre Bon, Shérazade Aknoun, Julien Savatier, Benoit Wattellier, Serge Monneret

Author Affiliations +

Proceedings Volume 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX; 858918 (2013) https://doi.org/10.1117/12.2003426
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

In this paper, we discuss the possibility of making tomographic reconstruction of the refractive index of a microscopic sample using a quadriwave lateral shearing interferometer, under incoherent illumination. A Z-stack is performed and the acquired incoherent elecromagnetic fields are deconvoluted before to retrieve in a quantitative manner the refractive index. The results are presented on polystyrene beads and can easily be expanded to biological samples. This technique is suitable to any white-light microscope equipped with nanometric Z-stack module.

Citation Download Citation

Pierre Bon, Shérazade Aknoun, Julien Savatier, Benoit Wattellier, and Serge Monneret "Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858918 (22 February 2013); https://doi.org/10.1117/12.2003426

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available