22 February 2013 Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope
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Abstract
In this paper, we discuss the possibility of making tomographic reconstruction of the refractive index of a microscopic sample using a quadriwave lateral shearing interferometer, under incoherent illumination. A Z-stack is performed and the acquired incoherent elecromagnetic fields are deconvoluted before to retrieve in a quantitative manner the refractive index. The results are presented on polystyrene beads and can easily be expanded to biological samples. This technique is suitable to any white-light microscope equipped with nanometric Z-stack module.
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Pierre Bon, Shérazade Aknoun, Julien Savatier, Benoit Wattellier, Serge Monneret, "Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope", Proc. SPIE 8589, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XX, 858918 (22 February 2013); doi: 10.1117/12.2003426; https://doi.org/10.1117/12.2003426
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