22 February 2013 Single-molecule imaging can be achieved in live obligate anaerobic bacteria
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Abstract
Single-molecule fluorescence (SMF) permits imaging with nanometer-scale resolution. This technique is particularly useful for cellular imaging as it provides a non-invasive, minimally perturbative means to examine macromolecular localization and dynamics, even in live cells. Here, we demonstrate that nanometer-scale SMF imaging can be extended to a new category of experiments: intracellular imaging of live, obligate anaerobic cells on the benchtop. We investigate the starch-utilization system (Sus) proteins in the gut symbiont Bacteroides thetaiotaomicron and discuss three different labels that we implemented to detect these proteins: fluorescent proteins, the tetracysteine-based FlAsH tag, and the enzymatic HaloTag.
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Krishanthi S. Karunatilaka, Krishanthi S. Karunatilaka, Ben R. Coupland, Ben R. Coupland, Elizabeth A. Cameron, Elizabeth A. Cameron, Eric C. Martens, Eric C. Martens, Nicole K. Koropatkin, Nicole K. Koropatkin, Julie S. Biteen, Julie S. Biteen, } "Single-molecule imaging can be achieved in live obligate anaerobic bacteria", Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 85900K (22 February 2013); doi: 10.1117/12.2004204; https://doi.org/10.1117/12.2004204
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