22 February 2013 New approach to double-helix point spread function design for 3D super-resolution microscopy
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Abstract
Double-helix point spread functions (DH-PSFs) have been used to extend localization and super-resolution microscopy to three dimensions. Current DH-PSF design provides a long depth-of-field for 3D imaging. However, it is not optimal for imaging under high background conditions. We present a method to design unconventional DH-PSFs with control on characteristics such as efficiency, transverse spread, and Fisher information. This allows tailoring the PSFs for specific applications. In particular, we introduce a design suitable for 3D localization-based super-resolution imaging under typical background conditions and demonstrate imaging with resolution one order of magnitude beyond the diffraction limit over a depth-of-field of 1.2 μm.
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Ginni Grover, Ginni Grover, Rafael Piestun, Rafael Piestun, } "New approach to double-helix point spread function design for 3D super-resolution microscopy", Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 85900M (22 February 2013); doi: 10.1117/12.2005694; https://doi.org/10.1117/12.2005694
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