SOFI of GABAB neurotransmitter receptors in hippocampal neurons elucidates intracellular receptor trafficking and assembly

Anja Huss; Omar Ramírez; Felipe Santibáñez; Andrés Couve; Steffen Härtel; Jörg Enderlein

doi:10.1117/12.2006215

22 February 2013 SOFI of GABA_B neurotransmitter receptors in hippocampal neurons elucidates intracellular receptor trafficking and assembly

Anja Huss, Omar Ramírez, Felipe Santibáñez, Andrés Couve, Steffen Härtel, Jörg Enderlein

Author Affiliations +

Proceedings Volume 8590, Single Molecule Spectroscopy and Superresolution Imaging VI; 85900N (2013) https://doi.org/10.1117/12.2006215
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The synaptic efficacy of neurons depends on the number of neurotransmitter receptors in the plasma membrane. The availability of these receptors is controlled by their specific intracellular trafficking routes. γ-Aminobutyric acid type B receptors (GABABRs) are heteromeric proteins consisting of GABA_BR1 and GABA_BR2 subunits. These receptors are found at the plasma membrane of somatodendritic postsynaptic sites and in axons. It is unknown whether the assembly of the subunits occurs directly in the somatic endoplasmic reticulum (ER) followed by vesicular transport, or whether the assembly occurs after the separate transport of the subunits to the dendritic ER compartment. To address this question we have studied the assembly of the GABA_BRs in hippocampal neurons with dual-color, 3D super-resolution optical fluctuation imaging (SOFI). SOFI is a fluorescence imaging modality which yields superresolved spatial resolution, 3D-sectioning and high image contrast. We will use the SOFI images to quantify the distribution of the GABA_BR subunits in the plasma membrane and in the dendritic intracellular compartments. Finally, we want to apply quantitative co-localization analysis to determine the compartments in which the assembly of the GABA_BR subunits occurs.

Citation Download Citation

Anja Huss, Omar Ramírez, Felipe Santibáñez, Andrés Couve, Steffen Härtel, and Jörg Enderlein "SOFI of GABA_B neurotransmitter receptors in hippocampal neurons elucidates intracellular receptor trafficking and assembly", Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 85900N (22 February 2013); https://doi.org/10.1117/12.2006215

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Receptors

Neurons

Plasma

Luminescence

Microscopy

Super resolution

Neurotransmitters

Show All Keywords

Keywords/Phrases

Search In:

Publication Years