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22 February 2013 Towards STED microscopy with nanometric optical sectioning
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Abstract
Circumventing the limit imposed by diffraction is a major issue in the instrumental development to realize finer resolutions in biological samples. With STED microscopy, we exploit the molecular transitions of the fluorescent marker to image well below the Rayleigh criterion. Also in combination with STED, we propose to use an alternative technique for optically sectioning fluorescent emitters close to the water-glass interface by selectively filtering the supercritical emission at the pupil plane. We discuss the instrumental development of such a system and its combination with other imaging techniques.
© (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Siddharth Sivankutty, Thomas Barroca, Guillaume Dupuis, Christophe Lefumeux, Céline Mayet, Arnaud Dubois, Catherine Marquer, Sandrine Lécart, Marie-Claude Potier, Emmanuel Fort, and Sandrine Lévêque-Fort "Towards STED microscopy with nanometric optical sectioning", Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 859018 (22 February 2013); https://doi.org/10.1117/12.2004883
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