22 February 2013 Towards STED microscopy with nanometric optical sectioning
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Circumventing the limit imposed by diffraction is a major issue in the instrumental development to realize finer resolutions in biological samples. With STED microscopy, we exploit the molecular transitions of the fluorescent marker to image well below the Rayleigh criterion. Also in combination with STED, we propose to use an alternative technique for optically sectioning fluorescent emitters close to the water-glass interface by selectively filtering the supercritical emission at the pupil plane. We discuss the instrumental development of such a system and its combination with other imaging techniques.
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Siddharth Sivankutty, Siddharth Sivankutty, Thomas Barroca, Thomas Barroca, Guillaume Dupuis, Guillaume Dupuis, Christophe Lefumeux, Christophe Lefumeux, Céline Mayet, Céline Mayet, Arnaud Dubois, Arnaud Dubois, Catherine Marquer, Catherine Marquer, Sandrine Lécart, Sandrine Lécart, Marie-Claude Potier, Marie-Claude Potier, Emmanuel Fort, Emmanuel Fort, Sandrine Lévêque-Fort, Sandrine Lévêque-Fort, } "Towards STED microscopy with nanometric optical sectioning", Proc. SPIE 8590, Single Molecule Spectroscopy and Superresolution Imaging VI, 859018 (22 February 2013); doi: 10.1117/12.2004883; https://doi.org/10.1117/12.2004883

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