Optical imaging of oxidative stress in retinitis pigmentosa (RP) in rodent model

Zahra Ghanian; Sepideh Maleki; Sandeep Gopalakrishnan; Reyhaneh Sepehr; Janis T. Eells; Mahsa Ranji

doi:10.1117/12.2004843

25 February 2013 Optical imaging of oxidative stress in retinitis pigmentosa (RP) in rodent model

Zahra Ghanian, Sepideh Maleki, Sandeep Gopalakrishnan, Reyhaneh Sepehr, Janis T. Eells, Mahsa Ranji

Proceedings Volume 8591, Optical Diagnostics and Sensing XIII: Toward Point-of-Care Diagnostics; 85910S (2013) https://doi.org/10.1117/12.2004843
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Oxidative stress (OS), which increases during retinal degenerative disorders, contributes to photoreceptor cell loss. The objective of this study was to investigate the changes in the metabolic state of the eye tissue in rodent models of retinitis pigmentosa by using the cryofluorescence imaging technique. The mitochondrial metabolic coenzymes NADH and FADH₂ are autofluorescent and can be monitored without exogenous labels using optical techniques. The NADH redox ratio (RR), which is the ratio of the fluorescence intensity of these fluorophores (NADH/FAD), was used as a quantitative diagnostic marker. The NADH RR was examined in an established rodent model of retinitis pigmentosa (RP), the P23H rat, and compared to that of control Sprague-Dawley (SD) rats and P23H NIR treated rats. Our results demonstrated 24% decrease in the mean NADH RR of the eyes from P23H transgenic rats compared to normal rats and 20% increase in the mean NADH RR of the eyes from the P23H NIR treated rats compared to P23H non-treated rats.

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Zahra Ghanian, Sepideh Maleki, Sandeep Gopalakrishnan, Reyhaneh Sepehr, Janis T. Eells, and Mahsa Ranji "Optical imaging of oxidative stress in retinitis pigmentosa (RP) in rodent model", Proc. SPIE 8591, Optical Diagnostics and Sensing XIII: Toward Point-of-Care Diagnostics, 85910S (25 February 2013); https://doi.org/10.1117/12.2004843

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KEYWORDS

Near infrared

Retina

Eye

Tissues

Eye models

Tissue optics

Animal model studies

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