Diabetes mellitus and hypertension diseases are frequently found in the same patient, which if untreated predispose to
atherosclerotic and kidney diseases. The objective of this study was to identify potential biomarkers in the urine of
diabetic and hypertensive patients through dispersive near-infrared Raman spectroscopy. Urine samples were collected
from patients with diabetes and hypertension but no complications (LG), high degree of complications (HG), and control
ones: one fraction was submitted to biochemical tests and another one was stored frozen (-20°C) until spectral analysis.
Samples were warmed up and placed in an aluminum sample holder for Raman spectra collection using a dispersive
spectrometer (830 nm wavelength, 300 mW laser power and 20 s exposure time). Spectra were then submitted to
Principal Components Analysis. The PCA loading vectors 1 and 3 revealed spectral features of urea/creatinine and
glucose, respectively; the PCA scores showed that patients with diabetes/hypertension (LG and HG) had higher amount
of glucose in the urine compared to the normal group (p < 0.05), which can bring serious consequences to patients. Also,
the PCA scores showed that the amount of urea decreased in the groups with diabetes/hypertension (p < 0.05), which
generates the same concern as it is a marker that has a strong importance in the metabolic changes induced by such
diseases. These results, applied to the analysis of urine of patients with diabetes/hypertension, can lead to early
diagnostic information of complications and a possible disease prognosis in the patients where no complications from
diabetes and hypertension were found.