Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique

Natasha Fairbairn; Rute Fernandes; Rachel Carter; Timothy J. Elliot; Antonios G. Kanaras; Otto L. Muskens

doi:10.1117/12.981941

22 February 2013 Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique

Natasha Fairbairn, Rute Fernandes, Rachel Carter, Timothy J. Elliot, Antonios G. Kanaras, Otto L. Muskens

Author Affiliations +

Proceedings Volume 8595, Colloidal Nanocrystals for Biomedical Applications VIII; 859501 (2013) https://doi.org/10.1117/12.981941
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We discuss a hyperspectral darkfield microscopy technique capable of imaging single nanoparticles at biologically compatible (<0.5 W/cm²) illumination conditions. The microscope was tested on an array of lithographically produced gold nanorod antennas and on colloidal gold nanorods deposited on a glass substrate. As a test for in-vitro imaging capabilities, we studied the uptake of gold nanorods by DC2.4 dendritic cells.

Citation Download Citation

Natasha Fairbairn, Rute Fernandes, Rachel Carter, Timothy J. Elliot, Antonios G. Kanaras, and Otto L. Muskens "Single-nanoparticle detection and spectroscopy in cells using a hyperspectral darkfield imaging technique", Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 859501 (22 February 2013); https://doi.org/10.1117/12.981941

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