22 February 2013 O6-alkylguanine-DNA transferase (SNAP) as capture module for site-specific covalent bioconjugation of targeting protein on nanoparticles
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Abstract
A bimodular genetic fusion comprising a delivery module (scFv) and a capture module (SNAP) is proposed as a novel strategy for the biologically mediated site-specific covalent conjugation of targeting proteins to nanoparticles. ScFv800E6, an scFv mutant selective for HER2 antigen overexpressed in breast cancer cells was chosen as targeting ligand. The fusion protein SNAP-scFv was irreversibly immobilized on magnetofluorescent nanoparticles through the recognition between SNAP module and pegylated O6-alkylguanine derivative. The targeting efficiency of the resulting nanoparticle against HER2-positive breast cancer cells was assessed by flow cytometry and immunofluorescence.
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Serena Mazzucchelli, Serena Mazzucchelli, Miriam Colombo, Miriam Colombo, Elisabetta Galbiati, Elisabetta Galbiati, Fabio Corsi, Fabio Corsi, Josè M. Montenegro, Josè M. Montenegro, Wolfgang J. Parak, Wolfgang J. Parak, Davide Prosperi, Davide Prosperi, } "O6-alkylguanine-DNA transferase (SNAP) as capture module for site-specific covalent bioconjugation of targeting protein on nanoparticles", Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 859502 (22 February 2013); doi: 10.1117/12.2001648; https://doi.org/10.1117/12.2001648
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