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22 February 2013Fluorescence Lifetime Imaging Microscopy (FLIM) of quantum dots in living cells
Fluorescence lifetime imaging microscopy (FLIM) is an emerging imaging technique that can indicate environmental
factors such as pH and redox potential by the effect of these factors on the fluorescence lifetimes of fluorophores.
Semiconductor quantum dots (QDs) are highly sensitive to environment and so are ideal for use in FLIM, although
certain experimental parameters must be carefully considered for QD imaging to account for their long lifetimes and
two-photon behavior. We image the uptake of three types of QDs in cultured fibroblasts and show some preliminary
results on the effects of endosomes and lysosomes on QD lifetimes. These results indicate the feasibility of FLIM for
studies using QDs in live cells.
Jay Nadeau andLina Carlini
"Fluorescence Lifetime Imaging Microscopy (FLIM) of quantum dots in living cells", Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 85950R (22 February 2013); https://doi.org/10.1117/12.2013379
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Jay Nadeau, Lina Carlini, "Fluorescence Lifetime Imaging Microscopy (FLIM) of quantum dots in living cells," Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 85950R (22 February 2013); https://doi.org/10.1117/12.2013379