Fluorescence Lifetime Imaging Microscopy (FLIM) of quantum dots in living cells

Jay Nadeau; Lina Carlini

doi:10.1117/12.2013379

22 February 2013 Fluorescence Lifetime Imaging Microscopy (FLIM) of quantum dots in living cells

Jay Nadeau, Lina Carlini

Author Affiliations +

Proceedings Volume 8595, Colloidal Nanocrystals for Biomedical Applications VIII; 85950R (2013) https://doi.org/10.1117/12.2013379
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

Fluorescence lifetime imaging microscopy (FLIM) is an emerging imaging technique that can indicate environmental factors such as pH and redox potential by the effect of these factors on the fluorescence lifetimes of fluorophores. Semiconductor quantum dots (QDs) are highly sensitive to environment and so are ideal for use in FLIM, although certain experimental parameters must be carefully considered for QD imaging to account for their long lifetimes and two-photon behavior. We image the uptake of three types of QDs in cultured fibroblasts and show some preliminary results on the effects of endosomes and lysosomes on QD lifetimes. These results indicate the feasibility of FLIM for studies using QDs in live cells.

Citation Download Citation

Jay Nadeau, Jay Nadeau, Lina Carlini, Lina Carlini, "Fluorescence Lifetime Imaging Microscopy (FLIM) of quantum dots in living cells", Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 85950R (22 February 2013); doi: 10.1117/12.2013379; https://doi.org/10.1117/12.2013379

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