Optical microscopy and spectroscopy of analyte-sensitive functionalized gold nanoparticles in microfluidic systems

Martinus H. V. Werts; Vincent Raimbault; Matthieu Loumaigne; Laurent Griscom; Olivier Français; Julien R. G. Navarro; Anne Débarre; Bruno Le Pioufle

doi:10.1117/12.2001608

22 February 2013 Optical microscopy and spectroscopy of analyte-sensitive functionalized gold nanoparticles in microfluidic systems

Martinus H. V. Werts, Vincent Raimbault, Matthieu Loumaigne, Laurent Griscom, Olivier Français, Julien R. G. Navarro, Anne Débarre, Bruno Le Pioufle

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Proceedings Volume 8595, Colloidal Nanocrystals for Biomedical Applications VIII; 85950W (2013) https://doi.org/10.1117/12.2001608
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

The use of microfluidic devices for the handling and analysis of suspensions of colloidal gold particles is presented. The plasmonic particles are detected via their resonant light scattering (RLS) in a simple and versatile LED-based dark field illumination geometry. RLS enables both microscopic imaging and microspectroscopy. The measurement of the diffusion coefficient of nanoparticles in this type of devices is demonstrated. Nanoparticles are separated from small (free ligand) molecules in a continuous flow process. Reversible aggregation of functionalized particles is detected in microflow by means of RLS, which opens perspectives for the development of microfluidic bioplasmonic detection schemes.

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Martinus H. V. Werts, Martinus H. V. Werts, Vincent Raimbault, Vincent Raimbault, Matthieu Loumaigne, Matthieu Loumaigne, Laurent Griscom, Laurent Griscom, Olivier Français, Olivier Français, Julien R. G. Navarro, Julien R. G. Navarro, Anne Débarre, Anne Débarre, Bruno Le Pioufle, Bruno Le Pioufle, "Optical microscopy and spectroscopy of analyte-sensitive functionalized gold nanoparticles in microfluidic systems", Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 85950W (22 February 2013); doi: 10.1117/12.2001608; https://doi.org/10.1117/12.2001608

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