22 February 2013 Fluorescent nanocolloids for differential labeling of the endocytic pathway and drug delivery applications
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The demonstration of fine control over nanomaterials within biological systems, particularly in live cells, is integral for the successful implementation of nanoparticles (NPs) in biomedical applications. Here, we show the ability to differentially label the endocytic pathway of mammalian cells in a spatiotemporal manner utilizing fluorescent nanocolloids (NCs) doped with a perylene-based dye. EDC-based conjugation of green- and red-emitting NCs to the iron transport protein transferrin resulted in stable bioconjugates that were efficiently endocytosed by HEK 293T/17 cells. The staggered delivery of the bioconjugates allowed for the time-resolved, differential labeling of distinct vesicular compartments along the endocytic pathway in a nontoxic manner. We further demonstrated the ability of the NCs to be impregnated with the anticancer therapeutic, doxorubicin. Delivery of the drug-doped nanoconjugates resulted in the intracellular release and nuclear accumulation of doxorubicin in a time- and dose-dependent manner. We discuss our results in the context of the utility of such materials for NP-mediated drug delivery applications.
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James B. Delehanty, James B. Delehanty, Christopher M. Spillmann, Christopher M. Spillmann, Jawad Naciri, Jawad Naciri, W. Russ Algar, W. Russ Algar, Banahalli R. Ratna, Banahalli R. Ratna, Igor L. Medintz, Igor L. Medintz, "Fluorescent nanocolloids for differential labeling of the endocytic pathway and drug delivery applications ", Proc. SPIE 8595, Colloidal Nanocrystals for Biomedical Applications VIII, 85951E (22 February 2013); doi: 10.1117/12.2007749; https://doi.org/10.1117/12.2007749

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