15 March 2013 ICG-loaded polymeric nanocapsules functionalized with anti-HER2 for targeted fluorescence imaging and photodestruction of ovarian cancer cells
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Abstract
Optical nano-materials present a promising platform for targeted molecular imaging of cancer biomarkers and its photodestruction. Our group is investigating the use of polymeric nanoparticles, loaded with indocyanine green, an FDA-approved chromophore, as a theranostic agent for targeted intraoperative optical imaging and laser-mediated destruction of ovarian cancer. These ICG-loaded nanocapsules (ICG-NCs) can be functionalized by covalent attachment of targeting moieties onto their surface. Here, we investigate ICG-NCs functionalized with anti-HER2 for targeted fluorescence imaging and laser-mediated destruction of ovarian cancer cells in vitro. ICG-NCs are formed through ionic cross-linking between polyallylamine hydrochloride chains and sodium phosphate ions followed by diffusion-mediated loading with ICG. Before functionalization with antibodies, the surface of ICG-NCs is coated with single and double aldehyde terminated polyethylene glycol (PEG). The monoclonal anti-HER2 is covalently coupled to the PEGylated ICG-NCs using reductive amination to target the HER2 receptor, a biomarker whose over-expression is associated with increased risk of cancer progression. We quantify uptake of anti-HER2 conjugated ICG-NCs by ovarian cancer cells using flow cytometery. The in-vitro laser-mediated destruction of SKOV3 cells incubated with anti-HER2 functionalized ICG-NCs is performed using an 808 nm diode laser. Cell viability is characterized using the Calcein and Ethidium homodimer-1 assays following laser irradiation. Our results indicate that anti-HER2 functionalized ICG-NCs can be used as theranostic agents for optical molecular imaging and photodestruction of ovarian cancers in-vitro.
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Baharak Bahmani, Yadir Guerrero, Valentine Vullev, Sheela P. Singh, Vikas Kundra, Bahman Anvari, "ICG-loaded polymeric nanocapsules functionalized with anti-HER2 for targeted fluorescence imaging and photodestruction of ovarian cancer cells", Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 859602 (15 March 2013); doi: 10.1117/12.2003453; https://doi.org/10.1117/12.2003453
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