Intracellular delivery of molecular beacons by PMMA nanoparticles and carbon nanotubes for mRNA sensing

A. Giannetti; S. Tombelli; C. Trono; M. Ballestri; G. Giambastiani; A. Guerrini; G. Sotgiu; G. Tuci; G. Varchi; F. Baldini

doi:10.1117/12.2007391

21 February 2013 Intracellular delivery of molecular beacons by PMMA nanoparticles and carbon nanotubes for mRNA sensing

A. Giannetti, S. Tombelli, C. Trono, M. Ballestri, G. Giambastiani, A. Guerrini, G. Sotgiu, G. Tuci, G. Varchi, F. Baldini

Author Affiliations +

Proceedings Volume 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V; 85960U (2013) https://doi.org/10.1117/12.2007391
Event: SPIE BiOS, 2013, San Francisco, California, United States

Abstract

We describe here the use of carbon nanotubes (CNTs) and polymeric nanoparticles made of a core of polymethylmethacrylate (PMMA) surrounded by a shell bearing cationic groups, as intracellular delivery tools of molecular beacons (MBs), particular fluorescent DNA probes, for the detection and localization of a specific mRNA. Survivin mRNA targeting MBs have been used with Atto647N and Blackberry 650 as fluorophore/quencher pair. The MB was anchored to the surface of CNTs and PMMA nanoparticles via a commercial sulfhydryl-reactive heterobifunctional crosslinker and the achieved nanomaterials were then characterized in vitro.

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A. Giannetti, S. Tombelli, C. Trono, M. Ballestri, G. Giambastiani, A. Guerrini, G. Sotgiu, G. Tuci, G. Varchi, and F. Baldini "Intracellular delivery of molecular beacons by PMMA nanoparticles and carbon nanotubes for mRNA sensing", Proc. SPIE 8596, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications V, 85960U (21 February 2013); https://doi.org/10.1117/12.2007391

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