21 February 2013 Dynamic SERS imaging with gold nanoparticles transported in a living cell
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Proceedings Volume 8597, Plasmonics in Biology and Medicine X; 859707 (2013) https://doi.org/10.1117/12.2005820
Event: SPIE BiOS, 2013, San Francisco, California, United States
Surface enhanced Raman scattering (SERS) has been used to detect biological molecules at a low concentration. We developed a rapid Raman imaging system, which can image dynamic activity of SERS agents, such as gold nanoparticles, in a living cell and the temporal behaviors of SERS spectra. Combination of slit scanning and an EM-CCD camera for measuring SERS spectra enables us to obtain a SERS image in a few seconds. The system can also be used to track a single particle moving in a cell with a laser focus and measure SERS spectra with a temporal resolution of 50 msec. By using the developed microscope systems, we monitored the change of SERS spectra associated cell transportation functions.
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Katsumasa Fujita, Katsumasa Fujita, Jun Ando, Jun Ando, Nicholas I. Smith, Nicholas I. Smith, Satoshi Kawata, Satoshi Kawata, "Dynamic SERS imaging with gold nanoparticles transported in a living cell", Proc. SPIE 8597, Plasmonics in Biology and Medicine X, 859707 (21 February 2013); doi: 10.1117/12.2005820; https://doi.org/10.1117/12.2005820

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