21 February 2013 Plasmon-enhanced emission from single fluorescent proteins
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Proceedings Volume 8597, Plasmonics in Biology and Medicine X; 85970J (2013) https://doi.org/10.1117/12.2002062
Event: SPIE BiOS, 2013, San Francisco, California, United States
In this work, we use evaporated gold nanoparticle films (GNPFs) as substrates for plasmon-enhanced imaging of two fluorescent proteins (FPs): mCherry and YFP. Through single-molecule epifluorescence microscopy, we show enhancement of single FP emission in the presence of GNPFs. The gold-coupled FPs demonstrate emission up to four times brighter and seven times longer lived, yielding order-of-magnitude enhancements in total photons detected. Ultimately, this results in increased localization accuracies for single-molecule imaging. Furthermore, we introduce preliminary results for enhancement of mCherry-labeled TcpP membrane proteins inside live Vibrio cholerae cells coupled to GNPFs. Our work indicates that plasmonic substrates are uniquely advantageous for super-resolution imaging and that plasmon-enhanced imaging is a promising technique for improving live cell single-molecule microscopy.
© (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jessica E. Donehue, Jessica E. Donehue, Beth L. Haas, Beth L. Haas, Esther Wertz, Esther Wertz, Courtney N. Talicska, Courtney N. Talicska, Julie S. Biteen, Julie S. Biteen, "Plasmon-enhanced emission from single fluorescent proteins", Proc. SPIE 8597, Plasmonics in Biology and Medicine X, 85970J (21 February 2013); doi: 10.1117/12.2002062; https://doi.org/10.1117/12.2002062

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