21 February 2013 Spectro-angular optical biosensor based on surface plasmon resonance operating in the visible spectrum
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Proceedings Volume 8597, Plasmonics in Biology and Medicine X; 859711 (2013) https://doi.org/10.1117/12.2004583
Event: SPIE BiOS, 2013, San Francisco, California, United States
Abstract
Surface plasmon resonance (SPR) sensing is one of the most widely used methods to implement biosensors due to its sensitivity and capacity for label-free detection. Most conventional SPR sensors measure the change in reflectance at a metal-dielectric interface as a function of either angle or wavelength. However, it has recently been shown that an increase in sensitivity and a greater robustness against noise can be achieved by measuring reflectivity in both domains simultaneously, in a so-called spectro-angular SPR biosensor. This provides a surface plasmon dispersion curve captured on an image sensor that can be tracked in real time. A single value decomposition method is used to project the dispersion curve onto a basis set and allow the image obtained from an unknown refractive index sample to be compared very accurately with a pre-calculated reference set. The objective of the current work is to further improve the detection limit of the spectro-angular biosensor. Simulations have shown that the spatial resolution and numerical precision of the image sensor have a significant impact on the accuracy of the refractive index change measurement. Therefore, upgrading the cameras used for the data acquisition could significantly improve the detection limit of the SPR biosensor. In this work, simulation results are presented to justify the modifications of the experimental system and to estimate the expected improvement in the detection limit of the spectro-angular biosensor by using higher spatial resolution and higher data precision cameras. Experimental results are presented and compared with the previous design.
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Sandrine Filion Côté, Sandrine Filion Côté, Philip J. R. Roche, Philip J. R. Roche, Andrew G. Kirk, Andrew G. Kirk, } "Spectro-angular optical biosensor based on surface plasmon resonance operating in the visible spectrum", Proc. SPIE 8597, Plasmonics in Biology and Medicine X, 859711 (21 February 2013); doi: 10.1117/12.2004583; https://doi.org/10.1117/12.2004583
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