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13 March 2013 IFSA: a microfluidic chip-platform for frit-based immunoassay protocols
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Point-of-care diagnostics (POC) is one of the key application fields for lab-on-a-chip devices. While in recent years much of the work has concentrated on integrating complex molecular diagnostic assays onto a microfluidic device, there is a need to also put comparatively simple immunoassay-type protocols on a microfluidic platform. In this paper, we present the development of a microfluidic cartridge using an immunofiltration approach. In this method, the sandwich immunoassay takes place in a porous frit on which the antibodies have immobilized. The device is designed to be able to handle three samples in parallel and up to four analytical targets per sample. In order to meet the critical cost targets for the diagnostic market, the microfluidic chip has been designed and manufactured using high-volume manufacturing technologies in mind. Validation experiments show comparable sensitivities in comparison with conventional immunofiltration kits.
© (2013) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Nadine Hlawatsch, Michael Bangert, Peter Miethe, Holger Becker, and Claudia Gärtner "IFSA: a microfluidic chip-platform for frit-based immunoassay protocols", Proc. SPIE 8615, Microfluidics, BioMEMS, and Medical Microsystems XI, 86150J (13 March 2013);

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