Effect of low level laser therapy and high intensity laser therapy on endothelial cell proliferation in vitro: preliminary communication

Malgorzata Lukowicz; Justyna Szymanska; Krzysztof Goralczyk; Andrzej Zajac; Danuta Rość

doi:10.1117/12.2012658

22 January 2013 Effect of low level laser therapy and high intensity laser therapy on endothelial cell proliferation in vitro: preliminary communication

Malgorzata Lukowicz, Justyna Szymanska, Krzysztof Goralczyk, Andrzej Zajac, Danuta Rość

Author Affiliations +

Proceedings Volume 8703, Laser Technology 2012: Applications of Lasers; 870303 (2013) https://doi.org/10.1117/12.2012658
Event: Tenth Symposium on Laser Technology, 2012, Szczecin, Poland

Abstract

Background: The main purpose of this study was to analyze the influence of power intensity and wavelength of Low Level Laser Therapy (LLLT) and HILT (High Intensity Laser Therapy) on endothelial cell proliferation.
Material and methods: The tests were done on human umbilical vein endothelial cells (HUVEC). Cultures were exposed to laser irradiation of 660 nm and 670 nm at different dosages, power output was 10 – 40 mW as well as 820 nm with power 100 mW and 808 nm with power 1500 mW. Energy density was from 0.28 to 11,43 J/cm². Cell proliferation of a control and tested culture was evaluated with a colorimetric device to detect live cells. The tests were repeated 8 times.
Results: We observed good effects of LLLT on live isolated ECs and no effects in experiments on previous deep-frozen cultures. Also HILT stimulated the proliferation of HUVEC.
Conclusion: Endothelial cells play a key role in vascular homeostasis in humans. We observed the stimulatory effect of LLLT and HILT on proliferation of HUVEC. Many factors influence the proliferation of EC, so is it necessary to continue the experiment with different doses, intensity and cell concentration.

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Malgorzata Lukowicz, Justyna Szymanska, Krzysztof Goralczyk, Andrzej Zajac, and Danuta Rość "Effect of low level laser therapy and high intensity laser therapy on endothelial cell proliferation in vitro: preliminary communication", Proc. SPIE 8703, Laser Technology 2012: Applications of Lasers, 870303 (22 January 2013); https://doi.org/10.1117/12.2012658

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