5 June 2013 Developing a cell-based sensor for the detection of Autoinducer-2
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Bacteria use an intricate set of communication systems for sensing and interpreting environmental cues that coordinate population-based behavior. Quorum sensing is one of these systems, and it involves the production, release, and detection of small chemical signaling molecules. Recent research has revealed the role of quorum sensing molecules in the control of microbial activities such as biofilm formation. In this presentation we outline the development of a recombinant E. coli cell-based sensor for detection of the quorum sensing molecule Autoinducer-2 (AI-2), as well as engineering strategies to remove sugar and anoxic inhibition of the strain.
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Matthew D. Servinsky, Matthew D. Servinsky, Katherine Germane, Katherine Germane, Elliot S. Gerlach, Elliot S. Gerlach, Chen-Yu Tsao, Chen-Yu Tsao, Christopher M. Byrd, Christopher M. Byrd, Christian J. Sund, Christian J. Sund, William E. Bentley, William E. Bentley, } "Developing a cell-based sensor for the detection of Autoinducer-2", Proc. SPIE 8719, Smart Biomedical and Physiological Sensor Technology X, 871903 (5 June 2013); doi: 10.1117/12.2016601; https://doi.org/10.1117/12.2016601

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