A new wireless detection device for the in-situ identification of Salmonella Typhimurium

Yating Chai; Howard C. Wikle; Mi-kyung Park; Shin Horikawa; Xie Hong; Bryan A. Chin

doi:10.1117/12.2015080

29 May 2013 A new wireless detection device for the in-situ identification of Salmonella Typhimurium

Yating Chai, Howard C. Wikle, Mi-kyung Park, Shin Horikawa, Xie Hong, Bryan A. Chin

Proceedings Volume 8721, Sensing for Agriculture and Food Quality and Safety V; 87210B (2013) https://doi.org/10.1117/12.2015080
Event: SPIE Defense, Security, and Sensing, 2013, Baltimore, Maryland, United States

Abstract

This paper presents a new device and method for the in-situ detection of Salmonella Typhimurium on tomato surfaces. This real-time in-situ detection was accomplished with phage-based magnetoelastic (ME) biosensors on fresh food surfaces. The E2 phage from a landscape phage library serves as the bio-recognition element that has the capability of binding specifically with S. Typhimurium. This mass-sensitive ME biosensor is wirelessly actuated into mechanical resonance by an externally applied time-varying magnetic field. When the biosensor binds with S. Typhimurium, the mass of the sensor increases, resulting in a decrease in the sensor's resonant frequency. Until now, ME sensors had to be collected from the tomato surface where they are exposed to S. Typhimurium and inserted into a measurement coil for the detection of the bacterium. In contrast, the newly designed test device allows the whole detection process to take place directly on the tomato. Changes in resonant frequency over time due to the accumulation of S. Typhimurium on the sensor were measured and are presented. Real-time in-situ detection of 20 minutes was achieved. In addition, this new methodology effectively decreases the measurement error and enables the simultaneous detection of multiple pathogens.

Citation Download Citation

Yating Chai, Howard C. Wikle, Mi-kyung Park, Shin Horikawa, Xie Hong, and Bryan A. Chin "A new wireless detection device for the in-situ identification of Salmonella Typhimurium", Proc. SPIE 8721, Sensing for Agriculture and Food Quality and Safety V, 87210B (29 May 2013); https://doi.org/10.1117/12.2015080

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available