Peptide-mediated cellular delivery of semiconductor quantum dots

Kelly Boeneman Gemmill; Markus Muttenthaler; James Delehanty; Jeff Deschamps; Kimihiro Susumu; Michael Stewart; Philip Dawson; Alan Huston; Igor Medintz

doi:10.1117/12.2015446

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29 May 2013 Peptide-mediated cellular delivery of semiconductor quantum dots

Kelly Boeneman Gemmill, Markus Muttenthaler, James Delehanty, Jeff Deschamps, Kimihiro Susumu, Michael Stewart, Philip Dawson, Alan Huston, Igor Medintz

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Proceedings Volume 8723, Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring III; 872311 (2013) https://doi.org/10.1117/12.2015446
Event: SPIE Defense, Security, and Sensing, 2013, Baltimore, Maryland, United States

Abstract

CdSe/ZnS semiconductor quantum dots (QDs) are ideal materials for biological sensing and cellular imaging applications due to their superior photophysical properties in comparison to fluorescent proteins or dyes and their ease of conjugation to biological materials. We have previously developed a number of in vitro FRET based biosensors in the laboratory for detection of proteases and biological and chemical agents. We would like to expand these biosensing capabilities into cellular systems, requiring development of QD cellular delivery techniques. Peptide mediated cellular delivery of QDs is ideal as peptides are small, easily conjugated to QDs, easily manipulated and synthesized, and can be designed with “handles” for further chemical conjugation with other cargo. Here we discuss four cell delivery peptides that facilitate QD uptake in live cells. Understanding these peptides will help us design better nanoparticle cellular delivery systems and advance our capabilities for in vivo biosensing.

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Kelly Boeneman Gemmill, Markus Muttenthaler, James Delehanty, Jeff Deschamps, Kimihiro Susumu, Michael Stewart, Philip Dawson, Alan Huston, and Igor Medintz "Peptide-mediated cellular delivery of semiconductor quantum dots", Proc. SPIE 8723, Sensing Technologies for Global Health, Military Medicine, and Environmental Monitoring III, 872311 (29 May 2013); https://doi.org/10.1117/12.2015446

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