Real-time polarimetric biosensing using macroporous alumina membranes

Jesús Álvarez; Laura Sola; Geoff Platt; Marina Cretich; Marcus Swann; Marcella Chiari; Daniel Hill; Juan Martínez-Pastor

doi:10.1117/12.2017432

28 May 2013 Real-time polarimetric biosensing using macroporous alumina membranes

Jesús Álvarez, Laura Sola, Geoff Platt, Marina Cretich, Marcus Swann, Marcella Chiari, Daniel Hill, Juan Martínez-Pastor

Author Affiliations +

Proceedings Volume 8765, Bio-MEMS and Medical Microdevices; 87650I (2013) https://doi.org/10.1117/12.2017432
Event: SPIE Microtechnologies, 2013, Grenoble, France

Abstract

We report the first demonstration of real-time biosensing in free standing macroporous alumina membranes. The membranes with their 200 nm diameter pores are ideal candidates for biosensing applications where fast response times for small sample volumes are needed as they allow analytes to flow through the pores close to the bioreceptors immobilized on the pores walls. A bulk refractive index sensitivity of 5.2x10^-6 refractive index units was obtained from signal responses to different concentrations of NaCl solutions flowing through the pores. Finally, after functionalizing the alumina pore surfaces with an epoxysilane and then spotting it with β-Lactoglobulin protein, the interactions between the β-lactoglobulin and rabbit anti-β-lactoglobulin, as well as the interaction between the rabbit anti-β-lactoglobulin and a secondary antibody anti-rabbit Immunoglobulin G were monitored in real-time.

Citation Download Citation

Jesús Álvarez, Laura Sola, Geoff Platt, Marina Cretich, Marcus Swann, Marcella Chiari, Daniel Hill, and Juan Martínez-Pastor "Real-time polarimetric biosensing using macroporous alumina membranes", Proc. SPIE 8765, Bio-MEMS and Medical Microdevices, 87650I (28 May 2013); https://doi.org/10.1117/12.2017432

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